Jute Material Coursework Example | Topics and Well Written Essays - 1250 words

Jute Material - Coursework Example Due to its resistive nature, the dry jute shows signs of being loaded after 23 milliseconds in the test. As indicated by the graph above the progress that follows soon after is represented by a shoot that is almost linear to the almost 2200 Newton’s mark after which the graph begins a drop, which creates a spike. The drop records a low of slightly above 1500 newton after which more force is applied to the jute where it spikes up to a force of 3300 newton. After which the drop follows. The drop is characterised by spikes resulting from the applied force as depicted in the graph in the twentieth and forty-sixth millisecond gap. The uncharacteristic drops in the loading process indicate a difference in dry jute that is uncharacteristic of the normal loading curve on the solid materials, and it reveals some characteristics of the dry jute.The test time that is recorded by the dry jute on the load increase is approximately 36 milliseconds after which the load is at the peak. The ov erall test records a time of approximately 46 milliseconds. From this, it is evident that the unloading or negative slope part of the graph takes less time compared to that of the loading part. The dry jute graph exhibits some unexpected results. Being a solid material, the graph that was expected on loading should have been characteristically smooth (Rees, 2000, p.123), but in this case, it had recorded instances and occurrence of sharp peaks in both the loading and unloading process.... 21), the fibre loses its elasticity element and enters the permanently deformed state. The fibre lingers shortly in the deformation phase, before suffering a complete breakdown in structure in this state. The breakpoint in the dry jute case is recorded at approximately 20.5 Joules. Wet Jute In construction, Jute is mostly used in its dry form. The presence of fluid in the internal structure of jute affects its mechanical properties as displayed in the graph. The effect of having the fluid in the jute is blown up when the material is put under high deformation forces. The wet jute displays similar properties in the initial test phases as depicted by the graph. The wet jute in this case is raw jute, which has not been pre-processed. It is harvested straight from the farms and used in this experiment. In the first stage of testing, the fibre shows resilience to the impact test forces just as the dry jute. It begins to show signs of deformation slightly before the twenty-third millisecon ds mark. After which it takes on elastic properties as the dry jute, with the graph being more linear-like when compared to the dry jute. The curve leading to permanent deformation in this case tends to arc more and its start point occurs earlier than that of dry jute. The breakpoint of wet jute is recorded at about 17 Joules. However, it takes about 40 milliseconds for the wet jute to get to the breaking point in the graph. Graph 2: Standard force Test time graph By applying standard force on the subjects, it is easy to establish the ultimate and yield stresses on the fibres. The dry and wet jute display different results in the experiment as indicated by Barsom and Rolfe (1999, p. 291). In conducting this experiment, the dry and wet jute

Company law Essay Example | Topics and Well Written Essays - 2500 words - 4

Company law - Essay Example For example, the verdict of this case law also highlights that the company can also be sued or can sue others as well. Through this authority, the company will be given an opportunity to defend itself and provide grounds for legitimising their business actions and their ramifications as well. At the same time, the company has right to own property, assets and liabilities as well. When these characteristics are compared with a natural human being, it can be deduced that both corporate entity and human being have similar rights, authority and ownership of assets. However, there are certain circumstances in which this artificial existence is not allowed but the veil of incorporation is lifted for ascertaining liability or any other legal issue that have necessitated such action. In the following parts of this essay, first the case study background has been provided in which the basic facts and other details have been included. Subsequently, the related held has also been provided. It is followed by various sections highlighting the influence on English company law and common law as well. In this discussion segment, both aspects have been highlighted so as to ascertain and understand the usefulness of this case law. For this purpose, support of subsequent other case laws has also been provided. Salomon was a merchant in the boot and leather business. With the passage of time, the business experienced a steady rise which required additional capital and members as well. In order to meet the needs of growing business, Solomon decided to form a limited company by taking his family members as partners. Subsequently, he sold the business control and ownership to the new company as the new company paid cash, debentures and shares as well. However, due to the certain events, the company was liquidated and the case was moved into the court because the parties argued that Solomon and the

Alcohol Dehydrogenase: From Ethanol To Acetaldehyde

Alcohol Dehydrogenase: From Ethanol To Acetaldehyde (20) 1. Alcohol dehydrogenase (AD) is an enzyme which catalyzes the reaction of its natural substrate ethanol to form acetaldehyde. The Km of AD, from rhinoceros livers, for ethanol is 1 X 10-3M. This enzyme is however somewhat non-specific and will recognize substrates other than ethanol. How would the kinetic plot be affected if AD were to separately catalyze methanol and isopropanol instead of ethanol? Assume that the overall Vmax remains the same in all 3 cases. How would the Km change for methanol compared to ethanol (higher, lower, the same)? How would the Km change for isopropanol compared to ethanol (higher, lower, the same)? How would the Kms of methanol and isopropanol compare (which higher than the other or about the same). Based upon your knowledge of the mechanisms by which enzymes work, briefly explain how you decided to place your new Kms. Hint: The alcohols are being added separately. There is not any kind of competition between the alcohols. They are not included in the same reaction. For your reference, the structures of these alcohols are below. Because ethanol is the natural substrate of Alcohol dehydrogenase (AD), AD would have a higher affinity and bind more readily to ethanol than other alcohols, including methanol and isopropanol. Because AD has a higher affinity for ethanol than other alcohols, its Km would be lower than methanol and isopropanol. The lower the Michaelis constant (Km) the less substrate required to get to  ½ Vmax or  ½ the maximum reaction rate and the higher the affinity of the enzyme for the substrate. Higher Km means more substrate concentration to reach  ½ Vmax and less affinity of the enzyme for the substrate. Vmax or the maximum reaction rate can be approached, but never actually reached. The Km for methanol would be higher than ethanol, thus requiring more substrate to reach  ½ Vmax and demonstrating lower affinity of AD for methanol. The Km for isopropanol would be higher than ethanol, thus requiring more substrate to reach  ½ Vmax and demonstrating lower affinity of AD for isopropanol . The Km for methanol would be lower than the Km for isopropanol and show a higher affinity for AD. The Michaielis-Menten kinetic plot would reflect a Km of 110-3M at  ½ Vmax for ethanol, a Km greater than 110-3M for methanol and a Km greater than the Km of methanol for isopropanol. The overall Vmax is the same for all three, so the  ½ Vmax for all three will stay the same. The plotted curve would become less vertical with the initial angle for ethanol becoming more acute and the curve becoming more linear as it changed from ethanol to methanol to isopropanol. Ethanol is ADs natural substrate, so based on enzyme mechanisms, it is able to bind more readily to AD due to its size and shape which fits ADs active site and allows ethanol to get close enough to create hydrogen bonds. The substrate and enzyme change confirmation and become destabilized which stabilizes the transition state, lowers the energy of activation and allows easier formation of the reaction products. Methanol and isopropanol do not bind as well, likely due to their structure or size and shape. Methanol is one carbon shorter which would prevent it from fitting in the AD site as well as ethanol and has fewer numbers of hydrogens, reducing H-bonding potential. Isopropanol is one carbon larger than ethanol which might make it too bulky to effectively bind to AD. Isopropanol is a secondary alcohol, with two carbon atoms attached to the carbon bonded to the OH, creating a bulky Y shape and not a chain alcohol like methanol and ethanol. This conformation and bulky shape prevents isopropanol from binding more readily than methanol, which is similar to ethanols linear shape. (10) 2. Briefly explain the protein cleavage involved in the maturation of an insulin molecule from proinsulin. Briefly explain 3 reasons why it is important that insulin be made as an inactive precursor requiring editing. Hint: Think in terms of things that would be important to the action of insulin (decreasing blood sugar). Protein cleavage is post-translational processing. Proinsulin is the precursor to insulin. Proinsulin is a polypeptide chain that loops around to form two disulfide bonds between four cysteine amino acids, two near either end. Endopeptidase cuts two molecules by proteolysis to remove the middle portion of the polypeptide. The final disulfide stabilized protein is insulin. Inactive proinsulin allows for optimal intracellular insulin stores that can be edited or activated quickly if needed to lower blood sugar and quickly prevent hyperglycemia. Proinsulins can be produced rapidly in response to elevated blood sugar with the post-translational processing switched off quickly; leaving the inactive molecules, once blood sugar is under control. Proinsulin is important because it is not degraded until it is needed, thus does not cause harmful low blood sugar levels and maintains sustained basal levels of insulin in the body. (10) 3. Briefly and individually outline the mechanisms of action for covalent, competitive, non-competitive, and uncompetitive enzyme inhibitors indicating how they effect enzyme action. For each type of inhibitor, describe a unique example of how we could learn something valuable, and at least somewhat practical, about an enzyme from each type of inhibitor study. The mechanism of action for covalent enzyme inhibitors is covalent binding in the enzyme active site and thus preventing substrate binding. This is irreversible and completely deactivates the enzyme requiring more enzymes to be produced to catalyze the reaction. This could tell us what amino acids bind in the enzyme active site by identifying covalent inhibitor modified functional groups and also substrate binding order. The mechanism of action for competitive enzyme inhibitors is they are shaped like the substrate and can bind in the enzyme active site, blocking the substrates binding. Competitive inhibitors can be outcompeted by increasing the substrate concentration and are reversible. Competitive inhibitors could be used to determine enzyme substrate affinities by finding out how much substrate is required and how long it takes to get back to  ½ Vmax. The mechanism of action for non-competitive enzyme inhibitors is they bind in a place other than the enzyme active site, allowing the substrate to bind, but they destabilize the transition state which hinders the enzyme by obstructing its proper performance and reducing Vmax. Non- competitive inhibitors are reversible, but cannot be outcompeted because they do not bind to the active site. Non-competitive inhibitors could be used to determine an enzymes induced fit mode of action as the substrate would still be able to bind, but not fully react. The mechanism of action for uncompetitive enzyme inhibitors is the substrate and inhibitor bind together in multi-substrate enzymes. While substrate binding and Km seem better, velocity is less because the inhibitor acts as part of the substrate. They are reversible. Uncompetitive inhibitors could be used to determine effective drug therapies by inhibiting an enzyme to varying degrees without permanently altering it, counter acting large amounts of the multi-substrate enzyme but not eliminating it from performing other useful functions. (10) 4. In discussing advances in molecular biotechnology, we mentioned 2 processes whose names sound remarkably similar called RFLP and AFLP. These two processes indeed share some similarities, but have many differences. Briefly explain 2 significant similarities that these share in their processes. Briefly explain 2 significant differences in terms of what these processes are used for. One similarity in RFLP and AFLP processes is cutting DNA for RFLP and cDNA for AFLP with restriction enzymes to create fragments. Another similarity is that DNA is electrophoresed in RFLP to separate different sized restriction fragments creating unique patterns for organisms or individuals (with the exception of twins) much like fingerprints and used for comparison. PCR products are electrophoresed in AFLP to compare tissues, experiments or expression profiling. One difference in what these processes are used for is RFLP is used to compare DNA from people or organisms for genetic fingerprinting and forensics, and AFLP is used to profile gene expressions (requiring mRNA to be converted to cDNA) of uncharacterized tissues, organisms or experiments. Another difference is AFLP can be used for Quantitative Trait Loci which help identify multifactorial inheritance of traits and assist in genome mapping, whereas RFLP is not used for QTL, but can be used for identifying a persons predisposition for a particular disease. (10) 5. Life on the planet Zornock encodes its genetic info in overlapping nucleotide triplets such that the translation apparatus shifts only one nucleotide at a time. In other words, if we had the nucleotide sequence ABCDEF on Earth this would be two codons (ABC DEF) whereas on Zornock it would be 4 codons (ABC, BCD, CDE, DEF) and the beginning of two others. Briefly explain and compare the effect of each of the following types of mutations on the amino acid sequence of a protein in 1) an earthling and 2) a Zornocker. A. The addition of one nucleotide. B. The deletion of one nucleotide. C. The deletion of 3 consecutive nucleotides. Assume these all occur in the middle of a gene. X = added nucleotide, ? = unknown nucleotide A1. One nucleotide added resulting in ABCXDEF in the earthling would create a frameshift that would produce the original codon ABC, a new codon XDE and one codon beginning F. A2. One nucleotide added resulting in ABCXDEF in the Zornocker would create one new codon, making a total of 5 codons, (ABC, BCX, CXD, XDE, DEF) and the beginning of two other codons EF? and F. B1. The deletion of one nucleotide resulting in ABCEF in the earthling would create a frameshift that would produce one original codon, ABC and two different beginnings EF? and F. B2. The deletion one nucleotide resulting in ABCEF in the Zornocker would result in 3 complete codons, ABC, BCE and CEF and two beginnings EF? and F. C1. The deletion of three consecutive nucleotides resulting in ABF in the earthling would create a frameshift that would result in one new codon, ABF. C2. The deletion of three consecutive nucleotides resulting in ABF in the Zornocker would result in one new codon and two partial codons, ABF and the beginnings BF? and F. The insertions and deletions in the earthling would produce a frameshift, creating different codons and a different polypeptide chain from the mutation on. Other effects of the frameshift could be inserting a different AA into the polypeptide or stopping translation altogether. These genotype effects could create non-functioning proteins or fragments, partially functioning proteins or no protein expression. The insertions and deletions in the Zornocker would add or remove codons at the site of the mutation, but would not alter the polypeptide chain after the mutation due to the overlapping nucleotide triplets. (10) 6. Imagine that weve isolated a new and potentially useful mutation in an existing model plant. Our goal as biotechnologists might be to characterize the mutation, figure out what protein it affects, figure out how it is expressed, figure out how it is controlled, and how to best take advantage of it for crop improvement. Using the techniques that weve covered so far, briefly outline a series of experiments and expected results, using at least 5 of the techniques weve discussed, to attempt to achieve the above goals. Hint: There is more than one way to do this. 1 In order to characterize the mutation, we could use Sanger DNA sequencing to determine the amino acid sequence of the mutated gene. We use a primer and DNA polymerase to start DNA synthesis. We then prepare reactions with dideoxynucleotides (ddNTP) for each nitrogenous base, A, T, C and G. We run the reactions with normal nitrogenous bases and one ddNTP nitrogenous base representing either A, T, C or G. The ddNTPs terminate the DNA chains and when all the reactions are electrophoresed on a gel with lanes A, T, C and G, we can read from the bottom up to determine the DNA sequence. We could then compare the DNA sequence to the sequence of the existing model plant to determine the differences in amino acid sequences caused by the mutation. 2. In order to characterize what protein it affects, we could detect gene expression and protein interactions by using qRT-PCR. First we create mRNA by transcribing the mutant DNA genes. Next, we convert the mRNA using reverse transcriptase to cDNA. Then we run a qPCR on the cDNA and add SYBR green to the products. SYBR green intercalates the DNA and we can measure the fluorescence and determine the number of mRNA copies, thus determining which proteins are affected. 3. In order to figure out how it is expressed, we could use DNA microarray and protein microarray analysis. With DNA microarrays we obtain gene chips and hybridize fluorescently labeled cDNA from the tissues containing the mutation. The mutation sample is compared to the model sample in parallel microarrays. A machine then analyzes and overlays the images to measure transcript levels, identify products and determine upregulation and downregulation of many proteins. We could also use protein microarrays which are similar to DNA microarrays, but are used to identify other proteins and compounds a protein interacts with. At times, protein function can be inferred by analyzing the environment in which it is expressed. 4. To figure out how it is controlled, we could use in situ hybridization to locate the mutant gene expression products or RNA molecules produced. First we chemically fix sample tissues to slides. With DNA probes we could localize mRNAs to see which cells and where in these cells the gene is being expressed. We could probe with antibodies to determine which proteins are being translated. We could add or subtract associated enzymes, substrates and cofactors and alter internal and external cell conditions to see how this changes the gene expression and thus determine how the gene is controlled. 5. To determine how best to take advantage of it, we could genetically engineer the model plant with the mutation by inserting the mutant DNA into a Ti plasmid, creating a recombinant Ti plasmid, and have Agrobacterium introduce that into the model plant. The Ti plasmid would recombine with the model plant DNA and create a genetically engineered plant that expresses the new trait. We could then run various experiments on the genetically engineered plant to determine if the trait is expressed as desired and if not, change the variables until we get the advantage we are looking for.

Essay --

Unit 3 Questions Directions: Completely outline each prompt. This is a typed assignment. Fill in each box completely. If it says do one or two of the following do all. For thesis statement just choose one point. 10 points off for every day late. 1. Analyze the changes and continuities in the Arabic world’s acceptance of ONE of the following items between 700 and 1400. Be sure to discuss the causes of the changes as well as the reasons for the continuities. Arts Technology Sciences Thesis with road map: DO NOT just repeat the prompt. Throughout this time period there has been changes and continuities among the Arab population acceptance of art. Mosques remained a focus of art most likely because much of them were firmly rooted in Islam. Break down each point. You need at least three pieces of evidence for each. Stay within the time period Arts A variety of different art styles emerged in as appreciation of finer art became more wide spread Elements of architecture were dispersed such as roman arches Mosques continued to be built throughout the Islamic Golden Age Technology Gunpowder was introduced to the middle east and began to develop in battle Irrigation technology was improved which lead to better yields Improved medical procedures epitomized by the breakthrough of modern surgery Sciences Superior mathematics allowed for advanced astronomical accuracy Mathematical concepts such as geometry and algebra were developed Throughout the time the Arabic number system was used Global Connection A Global connection in the Arabic Empire between 1400 and 700 was the creation of madrassas or centers of learning. Muslims Jews and Christians all came together to learn there. The best example of a Madras that connected the ... ...tionship Treatment of minority/indigenous religions Thesis with road map: DO NOT just repeat the prompt. Break down each point. You need at least three pieces of evidence for each. Stay within the time period Degree adoption of Islam Sub-Saharan Africa accepted it greatly Europeans fought Islamic expansion Military conflict The Crusades brought Europeans to try to reclaim the Holy Land Mansa Musa expanded his Islamic empire in West Africa Economic relationship European kingdoms traded widely with Mansa Musa’s kingdom for gold, and salt Islamic world taxed European goods traveling through the Middle East Treatment of minority/indigenous religion European kingdoms were intolerant of Islam, Jews and internal â€Å"heresies† Mansa Musa permitted a wide range of religions and fostered study of other religions and cultures Global Connection Other helpful information

Naturalistic Observation Essay

? Naturalistic Observation In order to study human development and perform naturalistic observation I went to the mall. I selected a 9-year-old white girl as the subject for my observation. I observed the subject for 30 minutes. During my observation the subject was not interrupted and was not aware of my study. The girl was spending time with her mother and brother at the mall. She was enjoying herself. She played some games with her brother. The games included jumping and running. During one of the games the girl had a short conversation with her brother. After that the girl started drawing pictures in her notebook. Mother of the subject had a very satisfied expression on her face when she saw the drawing her daughter made. During the whole observational period the girl looked relaxed and happy. The approximate height of the subject is 4ft 2in, the approximate weight is 70 lbs. She was well-dressed, her clothes were new and tidy. The girl was physically developed according to her age. During my whole observation the subject was very active, the girl was running and jumping, it was obvious that she had a good coordination. The child was also drawing pictures in her notebook, so I came to the conclusion that both her gross motor skills and fine manipulative skills were developed properly. Cognitive development of the subject was on a sufficient level. I observed the girl when she was buying an ice cream, she saw that one of the portions was on the bigger plate, but she chose one on the smaller plate because she liked the shape of it more. The girl was able to understand that if something changes in appearance it does not mean that the quantity of it changed. According to Piaget’s theory of cognitive development, the girl was on the concrete operational stage. It is the very important stage, because the child starts thinking logically and operationally. Being on this particular stage is absolutely adequate for children at the age of 9. The girl was emotionally and socially well developed. She obeyed the rules of the game and the rules of the mall while playing. During my observation the girl demonstrated that she was able to work in a team with her brother. She was friendly and well socialized. According to Erikson’s stages of social and emotional development this kind of behavior means that the girl was on the ‘school age’ stage. It is absolutely normal to be on this stage of social and emotional development at the age of 9. After my observation I came to the conclusion that the subject meets the milestones and stages for the physical, cognitive, social, and emotional development. Judging from the drawing that the girl made we can assume that the girl is gifted. According to my naturalistic observation there is nothing unusual in the development of the girl. According to her age she is absolutely adequate. The observation was a very interesting and informative process. It gave me the opportunity to apply different theories of development. The biggest advantage of this psychological method is the opportunity to observe the subject in the natural surrounding and watch his natural behavior. To sum up, during my naturalistic observation I applied Piaget’s theory of cognitive development and Erikson’s theory of emotional and social development. Both of these theories gave me the opportunity to understand the subject better. According to my observation, all physical skills of the girl were developed properly. After analysis I came to the conclusion that the subject is on the concrete operational stage of cognitive development, and on the ‘school age’ stage of social and emotional development. The girl is normally developed according to her age.

Typography and Clarendon

Clarendon is a classical font that was designed by Robert Besley. In 1842, Besley entered the design under Britain’s Ornamental Designs Act. It was than released in the year 1845 for the Fann Street Foundry in England. Clarendon was named after the Clarendon Press in Oxford. It is acknowledged as the first major listed English slab-serif typeface, because Besley worked hard at trying to patent the typeface. The font patents around that time merely lasted up to three years, as soon as it became extremely well liked and a lot of foundries copied it.In 1935, the Monotype foundry revised Clarendon typeface. The first templates and major hits for Clarendon stayed at Stephenson Blake and market and then afterwards founded at the Type Museum in London. In 1953, Hermann Eidenbenz took over and reworked it. Today Clarendon has ten font names that you can use such as: Clarendon Black, Clarendon Bold, Clarendon Extra Bold, Clarendon Bold Condensed, Clarendon Condensed, Clarendon Heavy, C larendon Light, Clarendon Medium, Clarendon Regular, and Clarendon Roman. They call it a slab-serif because it has some bracketing and some contrast in size.Clarendon was one of the final century typography to be developed. Clarendon is also a metal type and a popular wood type. It emphasizes text by being the primary design to be related bold. As an example of this it emphasizes, â€Å"WANTED† in the old west signs. Clarendon came along ways over the past years now several logotypes that are recognized in the United States of America uses this font. In 1973, the Sony logo was created and is used in Clarendon Bold Expanded. The national parks signs that are known worldwide are Clarendon font.Also People Magazine logo, Ruby Tuesday logo, Starbucks logo and so forth use the font Clarendon. Work Cited â€Å"Typedia. † : Clarendon. Media Temple, 2006-2012. Web. 24 Oct. 2012. . â€Å"Know Your Type: Clarendon. † Idsgn: A Design Blog. N. p. , 21 Aug. 2009. Web. 24 Oct . 2012. . SONY logo Clarendon in use: People magazine logo, US National Parks signage, Ruby Tuesday logo, Starbucks Chocolate logo, Pitchfork logo, Tonka logo, U2 album cover, Riki Watanabe clock, Wells Fargo logo

Free Essays on A Discussion Of “The Three-Stranded Allegory Of Moby-Dick”

In 1971, an article appeared entitled â€Å"The Three-Stranded Allegory of Moby-Dick was published by Allen Austin. The essay has as its purpose to familiarize the reader with the difference of character between Ahab and Ishmael in Moby-Dick. Herman Melville uses the main characters of Moby-Dick to begin the use of symbolism. Ahab believes that his material objects possess spiritual guidance. This belief causes him to believe his hunt will be successful. The idea of his hunt is based on this assumption. Ahab’s supernaturalism causes the reader to immediately grasp the difference in Ishmael. â€Å"Ahab pushes this idea of ‘intelligent malignity’ to the point of madness, accepting wholeheartedly the belief of the transcendentalists that the outward world is a symbol of spiritual reality.† Ishmael believes Ahab’s madness is caused by illumination. Ishmael comes to sympathize with Ahab’s view bud does not share in his beliefs. â€Å"Ishmael, in spite of a feeling of identity with Ahab, is convinced that man can expect only death from an indifferent universe.† Ishmael also believes Ahab will be a failure in his hunt for Moby Dick. Ahab believes Ishmael to be on the verge of madness. â€Å"Although he feels threatened by a wisdom that is woe, he feels threatened by a woe that is madness.† â€Å"The distinction between woe and madness parallels the distinction between Ahab’s and Ishmael’s attitudes toward Moby Dick.† â€Å"It represents the terror of death-manifested in the polar bear, the white shark, the White Squall, the marble pallor of the aspect of death, and the pallid hose on which Death rides.† The White Squall comes to symbolize to Ishmael the destructive principle at the beginning of existence. The world can not be happy with life’s disappointments, but creates a dream that drags out its repulse. â€Å"If as Ishmael says, significance lurks on all things, it is the significance of sothingness, of ... Free Essays on A Discussion Of â€Å"The Three-Stranded Allegory Of Moby-Dick† Free Essays on A Discussion Of â€Å"The Three-Stranded Allegory Of Moby-Dick† In 1971, an article appeared entitled â€Å"The Three-Stranded Allegory of Moby-Dick was published by Allen Austin. The essay has as its purpose to familiarize the reader with the difference of character between Ahab and Ishmael in Moby-Dick. Herman Melville uses the main characters of Moby-Dick to begin the use of symbolism. Ahab believes that his material objects possess spiritual guidance. This belief causes him to believe his hunt will be successful. The idea of his hunt is based on this assumption. Ahab’s supernaturalism causes the reader to immediately grasp the difference in Ishmael. â€Å"Ahab pushes this idea of ‘intelligent malignity’ to the point of madness, accepting wholeheartedly the belief of the transcendentalists that the outward world is a symbol of spiritual reality.† Ishmael believes Ahab’s madness is caused by illumination. Ishmael comes to sympathize with Ahab’s view bud does not share in his beliefs. â€Å"Ishmael, in spite of a feeling of identity with Ahab, is convinced that man can expect only death from an indifferent universe.† Ishmael also believes Ahab will be a failure in his hunt for Moby Dick. Ahab believes Ishmael to be on the verge of madness. â€Å"Although he feels threatened by a wisdom that is woe, he feels threatened by a woe that is madness.† â€Å"The distinction between woe and madness parallels the distinction between Ahab’s and Ishmael’s attitudes toward Moby Dick.† â€Å"It represents the terror of death-manifested in the polar bear, the white shark, the White Squall, the marble pallor of the aspect of death, and the pallid hose on which Death rides.† The White Squall comes to symbolize to Ishmael the destructive principle at the beginning of existence. The world can not be happy with life’s disappointments, but creates a dream that drags out its repulse. â€Å"If as Ishmael says, significance lurks on all things, it is the significance of sothingness, of ...